image lab 5 2 1 software (Bio-Rad)
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image lab 5 2 1 software
Image Lab 5 2 1 Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1605 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mac+software/Image+Lab+Software+for+Mac+Version+5%2E2%2E1/10__1038_slash_s41538___026___01184___w-231-28-32
Average 99 stars, based on 1605 article reviews
Image Lab 5 2 1 Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1605 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mac+software/Image+Lab+Software+for+Mac+Version+5%2E2%2E1/10__1038_slash_s41538___026___01184___w-231-28-32
Average 99 stars, based on 1605 article reviews
image lab 5 2 1 software - by Bioz Stars,
2026-10
99/100 stars
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seed extract and green-synthesized silver nanoparticles in a rat model of osteoporosis: possible TNF-α/RANKL modulation Article Snippet: Bands were detected using ClarityTM Western ECL Substrate (Cat. No. 170–5060, Bio-Rad, USA) and imaged with the ChemiDocTM MP Imaging System (Bio-Rad Laboratories, Hercules, CA, USA). .. Densitometric analysis was performed using Article Title: Profiling C‑Phycocyanin: From Extraction to Structure, IgE Recognition and Digestive Transformation of Spirulina’s Major Pigment‑Protein Article Snippet: .. The gels were stained with Coomassie Blue staining solution (G-250), further stained, following image acquisitionwithGelDocEZImager (Bio-Rad Laboratories, Inc., Hercules, CA, USA) or Bio-Rad ChemiDoc Imagers, and processed with Article Title: Nicotinamide nucleotide transhydrogenase (NNT) directly couples redox homeostasis to proline biosynthesis in human glioma Article Snippet: After the blocking step, membranes were incubated at 4°C overnight with the primary antibody of interest: anti-β-actin (Sigma-Aldrich A4700, 1:4000 in 5% BSA), anti-Cofilin (Cell Signaling, #5175, 1:2000 in 5% BSA), anti-NNT (Proteintech, 13442-2-AP, 1:500 in 5% BSA), anti-OAT (Santa Cruz, sc-374243, 1:1000 in 5% BSA), anti-ME3 (Abcam, ab172972 1:1000 in 5% BSA), anti-GLUD1 (Proteintech, 14299-1-AP, 1:1000 in 5% BSA), anti-IDH2 (Proteintech, 15932-1-AP, 1:1000 in 5% BSA), anti-VDAC (Cell Signaling, #4866, 1:1000 in 5% BSA), anti-ATP5A (Proteintech, 14676-1-AP, 1:1000 in 5% BSA), anti-β-Tubulin (Cell Signalling, #2146, 1:1000 in 5% BSA). .. After incubation with the specific secondary antibody - horseradish peroxidase-conjugated anti-mouse (Cell Signaling, 7076S) or anti-rabbit secondary antibodies (Cell Signaling, 7074S) for 1 hour, blots were developed using the chemiluminescence detection kit (Bio-Rad, 1705060) and acquired with a BIO-RAD ChemiDoc XRS+ imaging system and Article Title: Newcastle disease virus is a safe and immunogenic respiratory-delivered vaccine against SARS-CoV-2 in a ferret model Article Snippet: Membranes were probed with antibodies against SARS-CoV-2 spike S2 (NB100-56578, Novus Biologicals), NDV ribonucleoprotein (RNP; NBP2-11633, Novus Biologicals), and β-actin (MA5-15739, Invitrogen; cell lysates only). .. After application of the horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit IgG, G21234, Invitrogen; or goat anti-mouse IgG, G21040, Invitrogen), signal was detected using the SuperSignalTM West Pico PLUS Chemiluminescent substrate (Thermo Fisher, 34580), and imaged with a BioRad ChemiDoc MP Imaging system and BioRad Article Title: Systematic identification of permissive insertion sites for foreign gene expression in a NADC30-like PRRSV infectious clone and development of dual-fluorescent recombinant viruses. Article Snippet: Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen threatening the global swine industry.. Infectious clone and recombinant virus platforms serve as vital tools for investigating PRRSV biology and developing genetically engineered vaccines.. In this study, we constructed a full-length infectious clone of the NADC30-like PRRSV strain GX-2019 and systematically evaluated the tolerance of various genomic regions to foreign gene insertion. Article Title: Functional substitution of imatinib-inactivated canonical oncoprotein BCR::ABL1 by overexpressed PLAG1 results in BCR::ABL1-independent resistance in blast phase of chronic myeloid leukemia. Article Snippet: Inactivation of oncogenic BCR::ABL1 by imatinib in the early chronic phase (CP) of chronic myeloid leukemia (CML) has achieved unprecedented clinical success, which eludes the majority of terminal blast crisis (BC) patients, predominantly due to the scantily understood BCR::ABL1-independent mechanism/s of resistance.. Our earlier proteomic studies on K562 and KU812 cell lines, which mimicked the BCR::ABL1-independent resistance to imatinib reported in CML patients, demonstrated that atypical activation of signalling downstream of imatinib-inactivated BCR::ABL1, mediated by reduced levels of cell adhesion receptor integrin ITGB1, caused resistance.. In the present study, events upstream of ITGB1 were derived from the transcriptome, methylome, and miRNA profiles of imatinib-resistant cell lines. Control:Article Title: Anti-osteoporotic impact of
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seed extract and green-synthesized silver nanoparticles in a rat model of osteoporosis: possible TNF-α/RANKL modulation Article Snippet: Bands were detected using ClarityTM Western ECL Substrate (Cat. No. 170–5060, Bio-Rad, USA) and imaged with the ChemiDocTM MP Imaging System (Bio-Rad Laboratories, Hercules, CA, USA). .. Densitometric analysis was performed using Staining:Article Title: Profiling C‑Phycocyanin: From Extraction to Structure, IgE Recognition and Digestive Transformation of Spirulina’s Major Pigment‑Protein Article Snippet: .. The gels were stained with Coomassie Blue staining solution (G-250), further stained, following image acquisitionwithGelDocEZImager (Bio-Rad Laboratories, Inc., Hercules, CA, USA) or Bio-Rad ChemiDoc Imagers, and processed with other:Article Title: Mitochondrial transfer protects against cisplatin-induced hearing loss by fumarate-mediated activation of mitophagy. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Phosphatase Inhibitor Cocktail 2 Sigma‒Aldrich Cat#P5726 Phosphatase Inhibitor Cocktail 3 Sigma‒Aldrich Cat#P0044 CM5 sensor chip Cytiva Cat#BR100530 Recombinant PPP1CB protein Sino Biological Cat#P14-20G Recombinant PPP1CB protein Sangon Cat#D621143 Critical commercial assays Reactive Oxygen Species Assay Kit Beyotime Cat#S0033S GreenNucTM Caspase-3 Assay Kit for Live Cells Beyotime Cat#C1168M BeyoClickTM EdU Cell Proliferation Kit with AF555 Beyotime Cat#C0075S Pierce BCA Protein Assay Kit Beyotime Cat#P0011 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat#DC102-01 Mitophagy Detection Kit Dojindo Cat#MD01 EnzChek Phosphatase Assay Kit Thermo Fisher Scientific Cat#E12020 PierceTM Streptavidin Magnetic Beads Thermo Fisher Scientific Cat#88817 PureProteome Protein A/G Mix magnetic beads MeckMillpore Cat#LSKMAGAG02 Deposited data Bulk RNA-seq data files of SGNs This study GEO: GSE308431 Metabolomics data This study NMDR: PR003357 Experimental models: Cell lines Human: umbilical cord MSCs Jiangsu Renocell Biotech N/A Human: HEK293T cells ATCC Cat#CRL-3216; RRID: CVCL_0063 Rat: SGNs provided by Shandong Provincial ENT Hospital N/A Experimental models: Organisms/strains Mouse: C57BL/6J mice Vital River Laboratory Animal Technology Cat#219 Mouse: Ppp1cb-flox mice Gempharmatech Cat#T008169 Mouse: Pax2-Cre mice provided by Lin Gan, University of Rochester N/A Oligonucleotides Primers for qPCR, see Table S1 Sangon N/A Software and algorithms FlowJo, version 10.4 Tree Star www.flowjo.com GraphPad Prism, version 10.1.2 GraphPad Software www.graphpad.com Incubation:Article Title: Nicotinamide nucleotide transhydrogenase (NNT) directly couples redox homeostasis to proline biosynthesis in human glioma Article Snippet: After the blocking step, membranes were incubated at 4°C overnight with the primary antibody of interest: anti-β-actin (Sigma-Aldrich A4700, 1:4000 in 5% BSA), anti-Cofilin (Cell Signaling, #5175, 1:2000 in 5% BSA), anti-NNT (Proteintech, 13442-2-AP, 1:500 in 5% BSA), anti-OAT (Santa Cruz, sc-374243, 1:1000 in 5% BSA), anti-ME3 (Abcam, ab172972 1:1000 in 5% BSA), anti-GLUD1 (Proteintech, 14299-1-AP, 1:1000 in 5% BSA), anti-IDH2 (Proteintech, 15932-1-AP, 1:1000 in 5% BSA), anti-VDAC (Cell Signaling, #4866, 1:1000 in 5% BSA), anti-ATP5A (Proteintech, 14676-1-AP, 1:1000 in 5% BSA), anti-β-Tubulin (Cell Signalling, #2146, 1:1000 in 5% BSA). .. After incubation with the specific secondary antibody - horseradish peroxidase-conjugated anti-mouse (Cell Signaling, 7076S) or anti-rabbit secondary antibodies (Cell Signaling, 7074S) for 1 hour, blots were developed using the chemiluminescence detection kit (Bio-Rad, 1705060) and acquired with a BIO-RAD ChemiDoc XRS+ imaging system and Cell-Signaling:Article Title: Nicotinamide nucleotide transhydrogenase (NNT) directly couples redox homeostasis to proline biosynthesis in human glioma Article Snippet: After the blocking step, membranes were incubated at 4°C overnight with the primary antibody of interest: anti-β-actin (Sigma-Aldrich A4700, 1:4000 in 5% BSA), anti-Cofilin (Cell Signaling, #5175, 1:2000 in 5% BSA), anti-NNT (Proteintech, 13442-2-AP, 1:500 in 5% BSA), anti-OAT (Santa Cruz, sc-374243, 1:1000 in 5% BSA), anti-ME3 (Abcam, ab172972 1:1000 in 5% BSA), anti-GLUD1 (Proteintech, 14299-1-AP, 1:1000 in 5% BSA), anti-IDH2 (Proteintech, 15932-1-AP, 1:1000 in 5% BSA), anti-VDAC (Cell Signaling, #4866, 1:1000 in 5% BSA), anti-ATP5A (Proteintech, 14676-1-AP, 1:1000 in 5% BSA), anti-β-Tubulin (Cell Signalling, #2146, 1:1000 in 5% BSA). .. After incubation with the specific secondary antibody - horseradish peroxidase-conjugated anti-mouse (Cell Signaling, 7076S) or anti-rabbit secondary antibodies (Cell Signaling, 7074S) for 1 hour, blots were developed using the chemiluminescence detection kit (Bio-Rad, 1705060) and acquired with a BIO-RAD ChemiDoc XRS+ imaging system and Imaging:Article Title: Nicotinamide nucleotide transhydrogenase (NNT) directly couples redox homeostasis to proline biosynthesis in human glioma Article Snippet: After the blocking step, membranes were incubated at 4°C overnight with the primary antibody of interest: anti-β-actin (Sigma-Aldrich A4700, 1:4000 in 5% BSA), anti-Cofilin (Cell Signaling, #5175, 1:2000 in 5% BSA), anti-NNT (Proteintech, 13442-2-AP, 1:500 in 5% BSA), anti-OAT (Santa Cruz, sc-374243, 1:1000 in 5% BSA), anti-ME3 (Abcam, ab172972 1:1000 in 5% BSA), anti-GLUD1 (Proteintech, 14299-1-AP, 1:1000 in 5% BSA), anti-IDH2 (Proteintech, 15932-1-AP, 1:1000 in 5% BSA), anti-VDAC (Cell Signaling, #4866, 1:1000 in 5% BSA), anti-ATP5A (Proteintech, 14676-1-AP, 1:1000 in 5% BSA), anti-β-Tubulin (Cell Signalling, #2146, 1:1000 in 5% BSA). .. After incubation with the specific secondary antibody - horseradish peroxidase-conjugated anti-mouse (Cell Signaling, 7076S) or anti-rabbit secondary antibodies (Cell Signaling, 7074S) for 1 hour, blots were developed using the chemiluminescence detection kit (Bio-Rad, 1705060) and acquired with a BIO-RAD ChemiDoc XRS+ imaging system and Article Title: Newcastle disease virus is a safe and immunogenic respiratory-delivered vaccine against SARS-CoV-2 in a ferret model Article Snippet: Membranes were probed with antibodies against SARS-CoV-2 spike S2 (NB100-56578, Novus Biologicals), NDV ribonucleoprotein (RNP; NBP2-11633, Novus Biologicals), and β-actin (MA5-15739, Invitrogen; cell lysates only). .. After application of the horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit IgG, G21234, Invitrogen; or goat anti-mouse IgG, G21040, Invitrogen), signal was detected using the SuperSignalTM West Pico PLUS Chemiluminescent substrate (Thermo Fisher, 34580), and imaged with a BioRad ChemiDoc MP Imaging system and BioRad |